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library(monocle3)
library(Matrix)
library(Seurat)
library(SeuratWrappers)
plot_cells(p_sdata.cds, color_cells_by = "tumor.tag")
No trajectory to plot. Has learn_graph() been called yet?


p_sdata.cds <- order_cells(p_sdata.cds) # Choose cell(s) that you want to set as having pseudotime 0.
# Plotting by pseudotime
plot_cells(p_sdata.cds, color_cells_by = "pseudotime")
# The root nodes can also be selected programmatically - and given to order_cells by root_pr_nodes argument.
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c2VxdWVudGx5LCB1bmxpa2UgKktuaXQqLCAqUHJldmlldyogZG9lcyBub3QgcnVuIGFueSBSIGNvZGUgY2h1bmtzLiBJbnN0ZWFkLCB0aGUgb3V0cHV0IG9mIHRoZSBjaHVuayB3aGVuIGl0IHdhcyBsYXN0IHJ1biBpbiB0aGUgZWRpdG9yIGlzIGRpc3BsYXllZC4K